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KMID : 0380620190510040279
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Korean Journal of Food Science and Technology
2019 Volume.51 No. 4 p.279 ~ p.392
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Protective effect of Gabjubaekmok (Diospyros kaki) extract against amyloid beta (Aβ)-induced cognitive impairment in a mouse model
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Yoo Seul-Ki
Kim Jong-Min
Park Seon-Kyeong
Kang Jin-Yong
Han Hye-Ju
Park Hyo-Won
Kim Chul-Wo
Lee Uk
Heo Ho-Jin
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Abstract
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The current study investigated the effect of Gabjubaekmok (Diospyros kaki) ethanolic extract (GEE) on H2O2- induced human neuroblastoma MC-IXC cells and amyloid beta (Aβ)1-42-induced ICR (Institute of Cancer Research) mice. GEE showed significant antioxidant activity that was evaluated based on ABTS, DPPH scavenging activity, and inhibition of malondialdehyde (MDA) and acetylcholinesterase activity. Further, GEE inhibited ROS production and increased cell viability in H2O2-induced MC-IXC cells. Administration of GEE ameliorated the cognitive dysfunction on Aβ-induced ICR mice as evaluated using Y-maze, passive avoidance, and Morris water maze tests. Results of ex vivo test using brain tissues showed that, GEE protected the cholinergic system and mitochondrial functions by increasing the levels of antioxidants such as ROS, mitochondrial membrane potential (MMP), and adenosine triphosphate (ATP) against Aβ-induced cognitive dysfunction. Moreover, GEE decreasd the expression levels of apoptosis-related proteins such as TNF-α, p-JNK, p-tau, BAX and caspase 3. While, expression levels of p-Akt and p-GSK3β increased than Aβ group. Finally, gallic acid was identified as the main compound of GEE using high performance liquid chromatography.
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KEYWORD
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Gabjubaekmok, Diospyros kaki, Neuroprotective effect, Cognitive impairment, Apoptosis
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